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KMID : 0624620100430020140
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BMB Reports
2010 Volume.43 No. 2 p.140 ~ p.145
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High glucose induces differentiation and adipogenesis in porcine muscle satellite cells via mTOR
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Yue Tao
Yin Jing-Dong
Li Fengna
Li Defa
Du Min
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Abstract
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The present study investigated whether the mammalian target of rapamycin (mTOR) signal pathway is involved in the regulation of high glucose-induced intramuscular adipogenesis in porcine muscle satellite cells. High glucose (25 mM) dramatically increased intracellular lipid accumulation in cells during the 10-day adipogenic differentiation period. The expressions of CCAAT/enhancer binding protein-α (C/EBP-α) and fatty acid synthase (FAS) protein were gradually enhanced during the 10-day duration while mTOR phosphorylation and sterol-regulatory-element-binding protein (SREBP)-1c protein were induced on day 4. Moreover, inhibition of mTOR activity by rapamycin resulted in a reduction of SREBP-1c protein expression and adipogenesis in cells. Collectively, our findings suggest that the adipogenic differentiation of porcine muscle satellite cells and a succeeding extensive adipogenesis, which is triggered by high glucose, is initiated by the mTOR signal pathway through the activation of SREBP-1c protein. This process is previously uncharacterized and suggests a cellular mechanism may be involved in ectopic lipid deposition in skeletal muscle during type 2 diabetes.
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KEYWORD
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Adipocytes, CCAAT/enhancer binding protein-α, Lipid accumulation, mTOR signal pathway, Sterol-regulatory-elementbinding protein-1c, Type 2 diabetes
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